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SG Diagnostics

SG Diagnostics Cardiac Marker BNP Semi-Quantitative Detection Kit

This kit is intended for in vitro quantitative determination of B-type natriuretic peptide (BNP) in human serum, plasma, and whole blood samples.

BNP is a 32-amino acid peptide that is mainly synthesized and released by cardiac ventricles. Its levels correlate with ventricular pressure, amount of dyspnea, and state of neurohormonal modulation. BNP levels increase in proportion to ventricular volume expansion and pressure overload. It is a sensitive and specific indicator of changes in left ventricular function. Therefore, BNP tests can be used for diagnosing heart failure and assessing prognosis in patients with acute coronary syndrome (ACS).

This kit is a solid-phase immunochromatographic assay for the quantitative detection of BNP using the double antibody sandwich method. During testing, a sample (serum/plasma/whole blood) migrates upward by capillary action from the sample pad. On the conjugate pad, BNP in the sample forms quantum dot (QD)-labeled antibody-antigen complexes with antibody 1 (mouse anti-human BNP antibody-QD conjugate). The complexes are then captured by the test (T) line of antibody 2 (monoclonal mouse anti‑human BNP antibody) on the nitrocellulose membrane as they migrate with the sample, to form QD-labeled antibody-antigen-coated antibody immune complexes. 

The uncaptured QD-labeled complexes are then captured by the recombinant human BNP protein (E. coli) coated on the control (C) line as they continue to migrate upward, indicating the test is done properly. The QDs are excited to produce fluorescent signals, which are detected by SG Diagnostics Quantum Dot Fluorescence Analyzer to obtain a quantitative result. Within a certain range, fluorescence intensity varies linearly with the BNP concentration in the sample

Source: McFarlane, SI., (2003). “Role of the natriuretic peptide system in cardiorenal protection.” Arch Intern Med 163(22): 2696-2704.

The Quantum Dot Fluorescence Analyzer scans the test cassettes by activating the quantum dots with optical signals and receiving the optical signal re-emitted. The fluorescence intensity correlates with the concentration of analyte in the sample. The machine analyses the signal it received to give a semi-quantitative result of analyte concentration.

Source: edn.com

  • The assay time is shorter (within 10 minutes as compared to around 24 hours for enzyme-linked immunosorbent assay (ELISA))
  • The cost is cheaper than ELISA
  • It has a long shelf life and assay reagents do not require refrigeration or freezer storage
  • The samples do not need to be pre-treated before applying to the assay

Note: Read the Instructions for Use of the test kit and device carefully before operation. Bring the test strip to room temperature before use, and perform the test at room temperature

  1. Remove the test cassette from the aluminum foil pouch and place it on a horizontal dry surface.
  2. Dispense 80 μL of sample to the sample well of the test cassette.
  3. Depending on the selected mode, place the test cassette in the device. Results are displayed in 8 minutes.

Coming soon

  • Han Shiqin. Clinical Study of BNP Levels and Prognosis of Patients with Acute Coronary Syndrome [J]. The Medical Journal of Industrial Enterprise, 2009, 22 (1): 4-6
  • Zhou Jie, Zhang Bin, Zhao Yongyan, et al. Evaluation of Troponin T, Brain Natriuretic Peptide and Serum Sodium Levels on the Short-term Prognosis of Patients with Severe Chronic Heart Failure [J]. Jiangsu Medical Journal, 2015 (16):1946-1948
  • Bonow RO. New Insights into the Cardiac Natriuretic Peptides. Circulation 1996; 93:1946-1950

Coming soon

Test Kit Specifications Materials Provided

Test Cassettes (20)
A test cassette consists of a test strip, packed individually in a sealed desiccant aluminum foil pouch with a plastic dropper.
Product Insert (1)

Test Kit Characteristics

Assay format
Lateral flow test / immunochromatographic
Instrument
Quantum dot fluorescence analyser
Testing Time
10 -30 mins
Specificity
Sensitivity
Type of analyte
B-type natriuretic peptide (BNP)
Sample material
Whole blood, serum or plasma

External Clinical Evaluation
Coming soon

For more information, you can download the file below.