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SG Diagnostics

SG Diagnostics Cardiac Marker hs-cTnT Semi-Quantitative Detection Kit

This kit is intended for in vitro quantitative determination of cardiac troponin T (cTnT) in human serum, plasma, whole blood and peripheral blood samples.

Cardiac troponins (cTns) are regulatory proteins in myocardial muscle contraction. CTns are organized as troponin complex, which is composed of three different subunits: cardiac troponin T (cTnT), cardiac troponin I (cTnI), and troponin C (TnC). CTnT is stable, hydrophilic and reactive with specific antigenic determinants. It is a specific and highly sensitive marker for myocardial injury. 

When cTnT is present in the cytoplasm in its free form, it is released into the circulation as a result of increased cell membrane permeability due to ischemia/hypoxia-induced cardiomyocyte injury. The increase in cTnT level in blood is due to its release into the circulation 4-6 hours after cardiomyocyte injury. The increased level correlates to the severity of the myocardial injury. Therefore, cTnT is an important marker for the diagnosis and risk stratification of acute coronary syndrome (ACS).

Source: cytoskeleton.com

This kit is a solid-phase immunochromatographic assay for quantitative detection of cTnT using the double antibody sandwich method. During testing, a sample (serum/plasma/ whole blood/peripheral blood) migrates upward by capillary action from the sample pad. On the conjugate pad, cTnT in the sample forms quantum dot (QD)-labeled antibody-antigen complexes with antibody 1 (mouse anti-human cTnT antibody-QD conjugate). The complexes are then captured by the test (T) line of antibody 2 (monoclonal mouse anti ‑ human cTnT antibody) on the nitrocellulose membrane as they migrate with the sample, to form QD-labeled antibody-antigen-coated antibody immune complexes. The uncaptured QD-labeled complexes are then captured by the recombinant human cTnT protein (E. coli) coated on the control (C) line as they continue to migrate upward, indicating the test is done properly. The QDs are excited to produce fluorescent signals, which are detected by SG Diagnostics Quantum Dot Fluorescence Analyzer to obtain a quantitative result. Within a certain range, fluorescence intensity varies linearly with the cTnT concentration in the sample.

The Quantum Dot Fluorescence Analyzer scans the test cassettes by activating the quantum dots with optical signals and receiving the optical signal re-emitted. The fluorescence intensity correlates with the concentration of analyte in the sample. The machine analyses the signal it received to give a semi-quantitative result of analyte concentration.

Source: edn.com

  • The assay time is shorter (within 10 minutes as compared to around 24 hours for enzyme-linked immunosorbent assay (ELISA))
  • The cost is cheaper than ELISA
  • It has a long shelf life and assay reagents do not require refrigeration or freezer storage
  • The samples do not need to be pre-treated before applying to the assay

Note: Read the Instructions for Use of the test kit and device carefully before operation. Bring the test strip to room temperature before use, and perform the test at room temperature.

  1. Remove the test cassette from the aluminum foil pouch and place it on a horizontal dry surface.
  2. Dispense 80 μL of undiluted or diluted sample to the sample well of the test cassette. Note: Peripheral blood samples should be diluted to a ratio of 1:3 with normal saline (not provided). Dilution is not necessary for other type of samples.
  3. Depending on the selected mode, place the test cassette in the device. Results are displayed in 8 minutes.

Coming soon

  • Tang Xifan, Qin Xinling. Application of hs-cTnT Tests in the Diagnosis of Acute Myocardial Infarction [J]. The Journal of Practical Medicine, 2012, 28 (5): 828-829.
  • Zhang Shaojing, Wang Qing. Research Progress on the Influencing Factors of hs-cTnT [J].Beijing Medical Journal, 2016, 38 (2): 152-154.
  • Dominik G. Haider, Thomas Klemenz, Georg Martin Fiedler, et al. High sensitive cardiac troponin T: Testing the Test [J]. International Journal of Cardiology, 2017, 228: 779-783.

Coming soon

Test Kit Specifications Materials Provided

Test Cassettes (20)
A test cassette consists of a test strip, packed individually in a sealed desiccant aluminum foil pouch with a plastic dropper.
Product Insert (1)

Test Kit Characteristics

Assay format
Lateral flow test / immunochromatographic
Instrument
No
Testing Time
8 mins
Specificity
Sensitivity
Type of analyte
Cardiac Troponin T (cTnT)
Sample material
Whole blood, serum or plasma

External Clinical Evaluation
Coming soon

For more information, you can download the file below.